鸡朊样蛋白Doppel基因的克隆及其与PrPC相互关系分析

doi:10.11843/j.issn.0366-6964.2015.08.019

Abstract

Abstract:

This study aimed to clone the chicken Doppel protein (ChDpl) gene (PRND)，preliminary analyze its relationship with the normal cellular prion protein (PrP^C)，and to provide a theoretical and molecular basis for the research of avian Dpl structure and function，and the mechanism of the interaction relationship between Dpl and PrP^C.Based on the amino acid sequence of multi-species Dpl，the conserved region of Dpl amino acid sequence was found，and the degenerate primers and optimize primers were designed with chicken codon preference to reduce the degeneracy gradually.Chicken PRND gene was amplified by polymerase chain reaction (PCR) with the specific primers，and then was inserted into pMD-18-T vector.The sequence was uploaded to the GenBank database after identified.Besides，the relationship between Dpl and PrP^C was analyzed.Through the alignments of multi-species Dpl’s amino acid sequence，we found that the section 11-150 bits (140 amino acid residues) was the conserved region of Dpl’s amino acid sequence，by designing primers with this area and PCR amplification，we obtained about 417 bp target band，its GenBank accession number was KP140962.Through comprehensively analysis of the related researches of Dpl and PrP^C，we found that although Dpl and PrP^C have a similar post-translational modifications and spatial structure，they mostly show antagonism，namely PrP^C can antagonize the neurotoxicity of Dpl，especially the 23-88 th residues of its N-terminal peptide containing octapeptide repeat region are crucial for the protection of Purkinje cells from Dpl induced neurodegeneration.The successful cloning of avian Dpl not only fills the gaps of current researches，even more at the molecular level laid a foundation for further study of the structure and function of Dpl and its antagonistic mechanism with PrP^C.

CLC Number:

S852.659.7

ZHANG Tian-liang，WU Run，YANG Run-xia，WEI Jiao，WAN Xue-rui，LIU Xia，LIU Lei，ZHANG Xiao-li. Clone of Chicken Prion-like Doppel Gene and the Analysis of Its Interrelation with PrP^C[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1425-1431.

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References

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Clone of Chicken Prion-like Doppel Gene and the Analysis of Its Interrelation with PrP^C

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Clone of Chicken Prion-like Doppel Gene and the Analysis of Its Interrelation with PrPC

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Clone of Chicken Prion-like Doppel Gene and the Analysis of Its Interrelation with PrP^C